【摘要】 目的探讨丙戊酸在血管紧张素Ⅱ(AngⅡ)致心肌细胞肥大过程中的抑制作用。方法常规方法培养大鼠原代心肌细胞,分为3组:对照组、肥大组、丙戊酸组。利用AngⅡ刺激心肌细胞造成肥大模型,并给予丙戊酸进行干预。反转录聚合酶链反应观察β-肌球蛋白重链(-βMHC)mRNA表达;相差显微镜和电镜观察心肌细胞的表面积和超微结构变化;免疫组织化学法检测C-FOS蛋白的表达。结果原代心肌细胞在AngⅡ作用下表面积增加,超微结构发生改变;-βMHCmRNA和C-FOS蛋白表达增加(P<0.05)。给予丙戊酸干预后,上述变化显著缓解(P<0.05)。结论丙戊酸抑制AngⅡ刺激引起的心肌细胞肥大,为临床上治疗心肌肥厚提供一条新的思路。更多还原

【Abstract】 Objective To observe the inhibitory role of valproic acid(VPA) in hypertrophic cardiomycytes induced by angiotensin Ⅱ(AngⅡ)in rats.Methods The cultured rat cardiomyocytes were divided randomly into control group,hypertrophic group and VPA group.They were treated with AngⅡ to induce hypertrophy and then given with VPA.The morphologic changes of cardiomyocytes were observed under the contrast phase microscope and electron microscope.The mRNA levels of β-MHC were exmained by reverse transcriptase polymerase chain reaction(RT-PCR) method.The protein expression of C-FOS was exmanined by immunohistochemistry method.ResultsStimulated by AngⅡ,the cardiomyocytes were enlarged under the contrast phase microscope and the ultrastructure also changed.After stimulated by AngⅡ,the mRNA level of β-MHC and the protein expression of C-FOS increased in the hypertrophic cardiocytes.Given by VPA,these data decreased accordingly.Conclusion VPA may inhibit the cardiomyocyte hypertrophy induced by angiotensin Ⅱ in some degree.更多还原