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中药物质基础的高效液相色谱分离分析方法研究
The study of high-performance liquid chromatography methods for the separation and analysis of traditional chinese medicines
【摘要】 高效液相色谱方法已成为中药物质基础研究的重要手段.在中药质量控制、中药标准品制备、活性化合物发现等方面发挥着不可替代的作用.然而我们的实验数据表明,一个中药材可能包含上万个化合物.中药物质基础的复杂性对高效液相色谱方法提出了巨大挑战.本文针对液相色谱方法在中药物质基础分离分析中存在的问题与难点,结合红花、黄连、姜黄三味药材样品,从亲水色谱分离模式、新型色谱固定相、二维液相色谱分离系统和液相色谱质谱联用技术等方面讨论了液相色谱的分离分析方法和发展方向.结果表明,高效液相色谱新技术新方法在中药复杂体系的分离分析中具有很大的发展潜力和应用前景.
【Abstract】 The high-performance liquid chromatography (HPLC) methods have played an important role in the quality control of traditional Chinese medicines (TCMs), the preparation of standards of TCMs, the discovery of active compounds from TCMs and so on. Since, our data showed that there may be above ten thousands of compounds contained in a certain kind of TCMs, the separation and analysis of TCMs becomes a big challenge to the HPLC methods. This article discussed the problems and the development of the HPLC methods in the following aspects: hydrophilic interaction chromatography (HILIC) modes, novel HPLC stationary phase, two-dimensional HPLC separation system and high-performance liquid chromatography-mass spectrometry (HPLC-MS) method by investigating the extracts of Carthamus tinctorius L, Coptis chinensis Franch and Curcuma longa L. It shows that new technologies and new methods of HPLC have great potential and application prospects in the separation and analysis of TCMs.
【Key words】 the material basis of TCMs; HPLC-MS; chromatography stationary phase; HILIC; 2D-HPLC;
- 【文献出处】 中国科学(B辑:化学) ,Science in China(Series B:Chemistry) , 编辑部邮箱 ,2009年08期
- 【分类号】R284
- 【被引频次】32
- 【下载频次】3602