【摘要】 基于过氧化氢(H2O2)特异性催化水解磺酸酯,设计合成了新型绿色荧光探针:荧光素二磺酸酯(FS-1)和二氯荧光素二磺酸酯(FS-2)两种螺环内酯型化合物,用于活细胞内过氧化氢的检测.探针结构由元素分析、IR、1HNMR及13CNMR表征.实验表明:探针FS-1和FS-2在模拟生物体系中检测过氧化氢具有良好的选择性和灵敏度,且线性范围较宽.细胞成像显示:探针FS-1和FS-2用于PMA刺激或外加不同浓度H2O2孵育的小鼠腹膜巨噬细胞均呈现明亮的绿色荧光,且能对细胞内H2O2微摩尔级浓度变化产生响应,证明两探针均具有良好的膜渗透性、高的选择性及良好的灵敏度.该方法的建立对研究生物体内H2O2的产生,H2O2导致的各种疾病机制及H2O2介导的信号转导途径具有重要的理论及实际意义.更多还原

【Abstract】 Based on a mechanism of H2O2-mediated hydrolysis of sulfonates,two fluorescein disulfonates compounds (FS-1 and FS-2) are designed and synthesized as highly selective and sensitive fluorescent probes for imaging H2O2 in living cells,and the probes were characterized with elemental analysis,IR,1H NMR and 13C NMR. Upon reaction with H2O2,the probes exhibit strong fluorescence responses and high selectivity for H2O2 over other reactive oxygen species and some biological compounds. Furthermore,the sulfonate-based probes,as novel fluorescent reagents,are cell-permeable and can detect micromolar changes in H2O2 concentrations in living cells by using confocal microscopy.更多还原