【摘要】 目的研究多重耐药鲍曼不动杆菌(MDR-ABA)gyrA基因突变与环丙沙星耐药关系。方法以鲍曼不动杆菌gyrA基因序列为靶序列,用PCR+DNA测序等方法对62株临床分离株gyrA基因突变进行对比研究。结果在53株耐环丙沙星MDR-ABA菌中,有52株gyrA基因的83位表现出突变,其突变方式全为TCA→TTA,导致氨基酸丝氨酸→亮氨酸;8株环丙沙星敏感和1株环丙沙星中介的MDR-ABA菌gyrA基因无突变。结论临床分离的MDR-ABA菌对环丙沙星耐药的分子机制主要表现为gyrA基因83位氨基酸密码子突变。更多还原

【Abstract】 Objective To study the relationship between mutation of gyrA gene of multi-drug resistant Acinetobacter baumannii(MDR-ABA)and the resistance to ciprofloxacin.Methods With gyrA gene sequence of ABA as target sequence,mutation of gyrA gene of 62 strains of ABA were analyzed by using PCR and DNA sequencing.Results Among 53 strains of ABA which were resistant to ciprofloxacin,52 strains showed single point TCA→TTA mutation in the gyrA gene at codon 83.Leading to amino acid substitution of Ser→Leu.Eight strains of ABA which were sensitive to ciprofloxacin and 1 strain of ABA which was intermediary to ciprofloxacin showed no mutation.Conclusion The molecular mechanism of the resistant MDR-ABA clinically isolated was the mutation in gyrA gene at codon 83.更多还原