【摘要】 利用海藻酸钠/CaCl2凝胶包埋法固定转小鼠金属硫蛋白-Ⅰ(mMT-Ⅰ)基因聚球藻7002及其野生宿主藻,就固定化藻的生长、对重金属的耐受性及其去除重金属的性能进行了初步研究。结果表明,固定化藻细胞比游离藻细胞生长较慢,培养周期较长,且最大细胞浓度偏低,但固定化可有效提高藻细胞对重金属的耐受性。固定化转mMT-Ⅰ聚球藻对Pb,Cd和Hg的耐受系数(以每单位的细胞吸光值OD750计)分别为6.077,0.610,1.093 mmol/L,而固定化野生聚球藻对于Pb,Cd和Hg的耐受系数分别为1.981,0.170,0.091 mmol/L;固定化转mMT-I聚球藻对重金属的去除效能明显优于游离藻:经3 d的处理,固定化转MT藻对Pb,Cd,Hg的去除率分别为88.09%,81.23%,91.45%,而固定化野生藻在相同的条件下则分别为77.3%,73.47%,85.44%。更多还原

【Abstract】 The growth characteristics,tolerance to heavy metals and decontamination of heavy metals by transgenic Synechococcus sp.PCC 7002 with mouse metallothionein-Ⅰ(mMT-Ⅰ) gene entrapped in sodium alginate/CaCl2 gel were studied.The results show that although the immobilized transgenic cells grow slower than free transgenic ones,have a lower cell concentration and need a longer culturing time,its tolerance to heavy metals increases significantly.The tolerance of immobilized transgenic cells to Pb,Cd and Hg(based on cell absorbtion value OD750) is 6.077,0.61 and 1.093 mmol/L,whereas the tolerance of immobilized wild cells to Pb,Cd and Hg is 1.981,0.17 and 0.091 mmol/L.The decontaminating capacity of immobilized transgenic cells is obviously higher than free ones.After 3 days of absorption,the decontaminating percentage of Pb,Cd and Hg is 88.09%,81.23% and 91.45% respectively,whereas that of the immobilized wild cells is 77.3%,73.47% and 85.44%,respectively.The models developed for growth and decontamination of heavy metals by immobilized transgenic cells fit the experiment data well.更多还原