【摘要】 采用Hungate滚管法,从东北太平洋结壳区深海沉积物中分离纯化得到中度嗜盐、严格厌氧发酵细菌S191,通过扩增及测定其16S rDNA基因序列,利用"Clustalx"和"Mega"软件对该序列作同源性分析及系统发育分析,建立了嗜盐严格厌氧细菌属的系统发育树,表明菌株S191的16S rDNA基因序列与Halanarobium属菌株的最高同源性为98.5%,革兰氏染色呈阴性、直杆状、0.3~0.6μm×1.8~3.0μm、无运动、不产生芽孢,生长NaCl质量浓度为5%~25%,最适NaCl质量浓度为7.5%~10%,生长pH值6.0~8.9,最适pH值为7.8~8.0,生长温度15~42℃,最适温度为32~37℃,最佳条件下代时约3.2 h,初步鉴定为Halanarobium属内的一个新成员.更多还原

【Abstract】 Moderately halophilic,strictly anaerobic eubacteria S191 was isolated and purified from the sediment in Northeast Pacific deep-sea nodule province by using Hungate roll-tube technique,the 16S rDNA encoding gene of S191 was proliferated by PCR,the sequence was determined,and homology analysis was completed by "Clustalx" and "Mega" software,a phylogenetic tree of Halanaerobium was constructed,S191 is considered to be a new member of Halanaerobium.It is a Gram-negative,rod-shaped,non-motile,non-spore-forming obligate anaerobe that owns a cell size of 0.3-0.6 μm×1.8-3.0 μm.Growth was observed in the presence of 5%-25% NaCl,the optimum salt concentration for growth is 7.5%-10%.It grows at pH 6.0-8.9 and the optimum pH for growth is 7.8-8.0.It grows well at temperature 15-42 ℃,and the optimum temperature for growth is 32-37 ℃.The doubling time is about 3.2 h when grown at optimal condition.更多还原