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反相HPLC测定复方丹参片(无糖衣)中丹参酮Ⅱ_A的含量
The Determination of Tanshinone Ⅱ_A in the Compound Tanshen Tablet by HPLC
【摘要】 应用高效液相色谱法测定复方丹参片中丹参酮Ⅱ_A含量。采用HypersilODSC18色谱柱,流动相(甲醇82∶水18),流速1.0 mL.min-1,检测波长270 nm。测得丹参酮Ⅱ_A在0.46-2.8μg内线性良好,r=0.9996,平均回收率为98.88%,RSD=0.517%。本法为该药的生产提供了一种可行、快捷、准确、稳定质量控制途径。
【Abstract】 The content of Tanshinone Ⅱ_A in Compound Tanshen tablet was measured by HPLC.Chromatogram post Hyporsic ODSC18;methyl alcohol-water(82:18) as flow phone;flow rate 1.0 mL·min-1;Measure the wavelength 270 nm.Dehydrate create ester linear range in 0.46-2.8 μg,r =0.9996.The average rate of recovery is 98.88%,RSD=0.517%.This method can be used in the quality control of this medicine production.It is simple,convenient and reliable
- 【文献出处】 惠州学院学报(自然科学版) ,Journal of Huizhou University(Natural Science Edition) , 编辑部邮箱 ,2009年03期
- 【分类号】R286
- 【下载频次】102