【摘要】 目的运用流式细胞术检测二烯丙基二硫(diallyl disulfide,DADS)诱导人白血病HL-60细胞凋亡的作用。方法实验分对照组和DADS处理组,进行体外细胞培养,进行细胞形态学观察,应用流式细胞术检测凋亡率、早期凋亡细胞以及caspase-3活性。结果DADS诱导前后,HL-60细胞形态学发生明显改变,PI单染法检测结果:随着不同浓度DADS作用HL-60细胞24 h,HL-60细胞出现明显亚二倍峰,细胞周期也发生了相应的改变;Annexin V/PI双染法检测结果:10 mg/L DADS作用HL-60细胞4、8、12和24 h后,早期凋亡细胞显著增加,具有明显的时间依赖性(P<0.05);间接免疫荧光流式细胞术结果显示在DADS诱导HL-60细胞凋亡过程中有caspase-3的活化,且与作用时间呈依赖关系。结论流式细胞术检测结果表明,DADS能有效诱导HL-60细胞凋亡。更多还原

【Abstract】 Objective Use flow cytometry to detect diallyl disulfide inducing human leukemia HL-60 cells apoptosis. Methods HL-60 cells were treated with different concentrations of DADS.After incubation of HL-60 cells with DADS,the effects of DADS on HL-60 cell growth inhibition were estimated by morphology,flow cytometry with PI and Annexin Ⅴ/PI staining were used to determine apoptosis cells and the expression of actived caspase-3.Results After HL-60 cells were exposed to DADS,the typical apoptotic morphological changes were observed under the light microscope,after treated with different concentrations of DADS for 24 h,HL-60 cells presented obvious sub diploid peaks(P<0.05) and different cell cycle arrest.After treated with 10 mg/L DADS for 4,8,12,24 h,early apoptotic cells were greatly increased(P<0.05) and the expression of active caspase-3 was increased in a time-dependent manner.Conclusion Flow cytometry method show that DADS can efficiently induce human leukemia HL-60 cells apoptosis.更多还原