【摘要】 半滑舌鳎精子冷冻保存对于人工繁殖育苗、杂交育种、雌核发育及其性别控制研究具有重要的意义,为此,本文对半滑舌鳎精子冷冻保存方法进行了研究。分别利用2.8mol/L的二甲基亚砜(DMSO)、甘油(Gly)和1,2-丙二醇(PG)冷冻保存该鱼精子。结果显示,DMSO冷冻保存精子的活力较高。利用MPRS+2.8mol/L DMSO以1∶0.5、1∶1、1∶1.5和1∶2的比例稀释并冷冻精子,1∶1比例在冻前能够抑制精子的运动,冻后活力可达82.50±3.54%,显著高于其他稀释比例(P<0.05)。分别利用冷冻保存液A(MPRS+2.8mol/L DMSO)和B(TS-2+2.8mol/L DMSO)稀释平衡精子,精子在A中的冻前快速运动时间、寿命分别为37.75±6.45s和145.00±78.98s,与鲜精无显著差异(P>0.05)。利用以上两种冷冻稀释液冷冻保存精子,精子在A液中的冻后活力和寿命分别可达53.50±6.69%和98.00±13.51s,冷冻效果优于B液(P<0.05)。冷冻后精子的受精率和孵化率分别为55.00±5.00%和35.00±13.23%,受精率与鲜精无显著性差异(P>0.05),因此认为MPRS+2.8mol/LDMSO可用于半滑舌鳎精子的冷冻保存。更多还原

【Abstract】 Cryopreservation of tongue sole Cynoglossus semilaevis sperm offers benefits for artificial propagation,hybridization,gynogenesis and sex control.In order to develop a protocol to freeze tongue sole sperm,the effects of cryoprotectants,extenders and dilution ratio on motility,fertilization rate and hatching rate of frozen-thawed sperm were evaluated.Dimethylsulfoxide(DMSO)provided higher protection than glycerol and 1,2-propylene glycol during freezing and thawing.Motion time and life of sperm in A(MPRS +2.8 mol/L DMSO)and B(TS-2+2.8 mol/L DMSO)before freezing were 37.75±6.45 sec and 145.00±78.98 sec,which was not different from that of fresh sperm(P>0.05).The motility and life of frozen-thawed sperm in A were 53.50±6.69% and 98.00±13.51 sec,which was significantly higher than that of frozen-thawed sperm in B.When sperm were diluted with A at a ratio of 1∶0.5,1∶1,1∶1.5 and 1∶2,the highest motility(82.5±3.54%,1∶1 dilution ratio)of frozen-thawed sperm was obtained and which was significantly higher than that of other dilution ratio treatment.According to the results above,tongue sole sperm were cryopreserved successfully with MPRS+2.8 mol/L DMSO at a dilution ratio of 1∶1.As a result,the fertilization rate(55.0±5.0%)and hatching rate(35±13.23%)of frozen-thawed sperm were obtained,which was not significantly different from that of fresh sperm(P>0.05).更多还原