【摘要】 从渤海海泥样品中分离获得1株新型酯酶菌株,经鉴定为地衣芽孢杆菌Bacillus licheniformis。所得的MP-2酯酶的最适作用温度范围50～70℃,在60℃表现出了最高活性,属于耐热酶;最适作用pH为10,属于碱性酶,其pH值作用范围比较窄;具有良好的热稳定性;金属离子Co2+,Li+对酶具有激活作用,Ca2+对酯酶的活力没有显著影响,化学试剂SDS、EDTA及Tween-20对酯酶的抑制效果显著,对常见有机溶剂具有良好的耐受力;该酯酶对碳链长短不同的底物表现出不同的酶活。更多还原

【Abstract】 A novel marine esterase-producing bacteria isolated from the Bohai Sea sediment was identified as Bacillus licheniformis.The MP-2 esterase was primarily purified and characterized.The optimal range of temperature of the esterase was 50～70 ℃,and the maximum activity was achieved at 60 ℃ and pH10.0.The esterase was alkaline and its optimum range of pH was narrow.The thermal stability of the esterase was good.Co2+,Li+ions were found to activate the esterase,but Ca2+ had no significant effects on the activity of the esterase.While SDS,EDTA and Tween-20 had significantly suppressive effects on the esterase.It showed strong resistance to ordinary organic solvents.The enzyme exhibited different esterase activities towards substrates with different length of carbon chains.更多还原