【摘要】 采用16S rDNA特异引物对63F和842R对蛭弧菌标准菌株Bdellovibrio bacteriovorus 109-J和实验菌株Bd-9913进行了PCR鉴定,使用Bd-9913菌株分别对3株虾类病原菌溶藻弧菌Vibrio alginolysticus LS01、HF09,副溶血弧菌V.parahaemolyticusV28以及1株大肠埃希氏菌Escherichiacoli CH-42进行裂解。结果表明,105PFU/ml的Bd-9913对于109CFU/ml的宿主菌具有较好的裂解效果。采用3.0×105PFU/ml Bd109-J和Bd-9913分别与饲料混合(v/w)后投喂3.0g左右的凡纳滨对虾幼虾,14d内未发现对虾异常,且在对虾肠道内检测不到Bd109-J及Bd-9913,证明二者对于凡纳滨对虾幼虾是安全的。对正常养殖了50d的室外集约化虾池水体分别泼洒终浓度0.3mg/L的二氯异氰脲酸、0.3mg/L季胺盐络合碘和3.0×105PFU/ml蛭弧菌,后者对于抑制水体弧菌活菌浓度的效果明显优于化学消毒剂。当每隔20d左右分别进行一次上述水体处理,通过比较体内弧菌感染的对虾个体数,发现从肝胰腺分离到弧菌的对虾数量二者无显著差异,但从血淋巴分离到弧菌的对虾数量,蛭弧菌处理组显著少于化学消毒剂处理组,显示了蛭弧菌在预防对虾弧菌感染方面的优越性。更多还原

【Abstract】 Bdellovibrio bacteriovorus strain Bd-9913 was identified using 16S rDNA universal primers 63F and 842R. The lytic ability of Bd-9913 on shrimp pathogenic bacterial agents, Vibrio alginolysticus and V. parahaemolyticus, and a standard strain of Escherichia coli was investigated respectively. The safety of Bd109-J and Bd-9913 to postlarvae of white leg shrimp Litopenaeus vannamei,was demonstrated in two weeks after oral administration of 3.0 105 PFU/ml Bdellovibrios mixed 1∶1(v/w) with commercial feed. Disinfection effect of Vibrioes in shrimp water was compared between chemical disinfectant treatment (0.3mg/L chloride and/or iodide) and Bdellovibrios addition (3.0×105 PFU/ml). Results indicated that the later showed great advantage to the former in keeping the concentration of live vibrios at a safe level twice as long (20 days). Furthermore,advantage was also shown when Bdellovibrios rather than chemical treatment was used to control shrimp vibrio infection in the outdoor intensive shrimp ponds.更多还原