节点文献
Cu2+和Zn2+对日本新糠虾生长、蛋白含量和体内磷酸酶活性的影响
Effects of Cu2+ and Zn2+ on growth and activity of phosphatase of Neomysis japonica Nakazawa
【摘要】 观察了不同浓度(10、20、40和80μg/L)重金属离子Cu2+和Zn2+对日本新糠虾存活、蜕皮、蛋白含量和体内磷酸酶(碱性磷酸酶和酸性磷酸酶)活性的慢性(30 d)影响。结果显示:(1)Cu2+和Zn2+能明显降低糠虾存活率,金属离子浓度增高,存活率降低,其中Cu2+引起的存活率降低更为明显,在最高浓度(80μg/L)Cu2+组中糠虾的存活率仅为同浓度Zn2+组的1/3,两组均明显低于对照组(P<0.05);Cu2+和Zn2+能明显降低糠虾蜕皮次数,最高浓度(80μg/L)Cu2+和Zn2+中糠虾的蜕皮数分别为11.00±1.73(次)和11.33±0.58(次),分别约为对照组的55.92%和57.6%。(2)Cu2+和Zn2+均能明显影响糠虾蛋白含量,其中Cu2+的影响力更强,最高浓度(80μg/L)Cu2+组糠虾体内蛋白白含量占湿重比为9.78%,仅约为对照组的1/2。(3)Cu2+和Zn2+能明显抑制糠虾体内两种磷酸酶的活力,碱性磷酸酶和酸性磷酸酶活力能作为糠虾监测环境重金属污染(Cu2+和Zn2+)的检测指标。
【Abstract】 The effects of different concentration Cu2+ and Zn2+(10,20,40 and 80 μg/L)on the growth,the protein content and the activity of phosphatase of the Neomysis japonica(Nakazawa)were studied by feeding experiment in aquarium of Laboratory for 30 d.The results showed as follows:(1) The survival of mysids was obviously lower in Cu2+ and Zn2+ groups than in control group(P<0.05),especially in Cu2+ group.The survival in most highest concentration Cu2+ group(80 μg/L) was only 1/3 in same concentration Zn2+ group;Compared with the control group,molt number of mysids in Cu2+ and Zn2+ groups obviously decreased(P<0.05).(3) The activities of phosphatase(Alkaline phosphatase and Aaciphosphatase) were decreased after Cu2+ and Zn2+ treated.The activities of phosphatase in mysids could be used to monitor Cu2+ and Zn2+ pollution.
【Key words】 Neomysis japonica Nakazawa; Cu2+; Zn2+; growth; phosphatase;
- 【文献出处】 海洋环境科学 ,Marine Environmental Science , 编辑部邮箱 ,2009年06期
- 【分类号】S917.4
- 【被引频次】12
- 【下载频次】226