【摘要】 从小球藻生长及细胞微观结构方面对比研究了纳米NiO与Ni2+对小球藻(Chlorellasp.)的胁迫性。结果表明概率单位法计算得纳米NiO对小球藻96 hEC50为31.4 mg/L,而Ni2+对小球藻96hEC50为1.73 mg/L,利用扫描电子显微镜、透射电子显微镜研究了受纳米NiO和Ni2+胁迫前后小球藻细胞微观结构变化,结果表明,受Ni2+胁迫的小球藻多数出现细胞缩小、变形、细胞器损坏、空泡等细胞凋亡现象,而受纳米NiO胁迫的细胞则部分表现细胞凋亡现象,表明Ni2+对小球藻的毒性远大于纳米NiO。更多还原

【Abstract】 The growth condition and micro-morphology were studied to reveal the inhibition of NiO nanoparticles to Chlorella sp.by contrast with Ni2+.The EC50 value of NiO nanoparticles to Chlorella sp.at 96 h was 31.4 mg/L by the probability unit method,but the EC50 value of Ni2+ was 1.73 mg/l in the same condition.The cell micro-morphology of Chlorella sp.inhibited by NiO and Ni2+ were observered by Scanning Electron Microscopy(SEM) and Transimission Electron Microscopy(TEM).It showed that the cell of Chlorella sp.inhibited by Ni2+ was shrinked,distored and the cell organ was destroyed.However,the one inhibited by NiO partly showed a phenomenon of the cell languishment.The result showed that Ni2+ was far more toxic than NiO nanoparticles.更多还原