【摘要】 应用垂直板聚丙烯酰胺凝胶电泳(PAGE)技术,对分别取自青岛、日照、荣成、长岛的野生黑鲪及荣成黑鲪人工繁育的F1代共5个群体的等位酶多态性进行分析。结果表明,分析的5个群体13种等位酶的25个基因座位中,多态位点比例(P)为36.0%—20.8%,平均每个位点的预期杂合度(He)为0.076—0.036,平均每个位点的实际杂合度(Ho)为0.063—0.040,证明所选5个群体具有较高的遗传多态性。5个群体之间遗传距离(D)为0.024—0.001,根据遗传距离进行UPGMA聚类分析,5个群体聚类次序与亲缘关系和地理位置表现出明显的正相关关系,长岛群体与其它四个群体间遗传距离最大(0.024—0.020),达到居群间差异水平。更多还原

【Abstract】 Allozymes of five populations of black rockfish Sebastes schtegeli collected from the coastal areas of Shandong Peninsula are isolated and analyzed with polyacrylamid gel electrophoresis (PAGE). 25 loci in 13 enzymes are examined. Result shows that the mean proportion of polymorphic loci (P) ranges 36.0%—20.8%, predicted heterozygosity (He) 0.076—0.036, and actual heterozygosity (Ho) 0.063—0.040, showing a large genetic diversity among the 5 populations with the genetic distance (D) from 0.024 to 0.001. The result of UPGMA shows that the cluster reflects a very positive correlation among populations and locations. In terms of genetic distance, Changdao population is far from other 4 populations, and reaches the level of different geographical population.更多还原