【摘要】 目的研究不同气腹介质对人结肠癌细胞株LS-174T在体外实验中增殖情况的影响,探讨腹腔镜应用于结直肠癌手术的安全性与可行性。方法MTT法测定细胞增殖数的情况。软琼脂克隆形成试验测定单个细胞增殖能力。免疫组织化学检测HIF-1α、VEGF的表达。结果CO2气腹作用后人结肠癌细胞LS-174T增殖速度高于对照组(P<0.05),CO2、He人工气腹作用后结肠癌细胞中HIF-1α和VEGF的阳性表达率高于对照组(P<0.01)。结论CO气腹可促进结肠癌细胞LS-174T的生长,He气腹相对抑制其生长。更多还原

【Abstract】 Objective To discuss the safety and feasibility of artificial pneumoperitoneum in the colon carcinoma operation through the investigation about the influence of different pneumoperitoneal medium on colon carcinoma LS-174T cell proliferation in vitro.Methods The cell proliferation condition was measured by MTT.Single cell proliferational capacity was tested by soft agar clone formation experiment.The expression of HIF-1α and VEGF were examined by immunohistochemistry.Results The growth speed and proliferating capacity of colon carcinoma cell LS-174T after CO2 pneumoperitoneum effect were higher than control group(P<0.05).Positive expression rates of HIF-1α and VEGF in colon carcinoma cell affected by CO2 and He artificial pneumoperitoneum were higher than control group(P<0.01).Conclusion CO2 pneumoperitoneum can facilitate the growth of colon carcinoma cell LS-174T,while He pneumoperitoneum can inhibit its growth relatively.更多还原