【摘要】 目的探讨苏木醇提单体原苏木素A对心脏移植后大鼠T淋巴细胞亚群的免疫抑制和对心肌的保护作用。方法应用大鼠腹腔同种异位心脏移植模型。实验分为原苏木素A组、环孢素组、对照组A(手术不给药组)、对照组B(不给药不手术组)。术前2天给药,术后第7天采血,应用流式细胞仪检测外周血T淋巴细胞亚群的变化,并检测心肌组织的病理改变。结果与心脏移植对照组比较,原苏木素A组、环孢素A组术后3天和7天时均减轻心脏移植后急性排斥反应所引起的心肌病理损害(P<0.01);与对照组B相比较,对照组A CD4+/CD8+明显升高(P<0.01),环孢素A组、原苏木素A组则可抑制CD4+/CD8+的升高。结论苏木醇提单体原苏木素A对心脏移植后发生的急性免疫排斥反应有明显的抑制作用,对心肌有明显的保护作用。更多还原

【Abstract】 Objective To discuss the effect of protosappanin A(one of ethanol extract of Caesalpinia Sappan L)on the change of T lymphocyte subsets and its protective effect on myocardial tissues after allograft rat heart transplantation.Methods The cardic allograft rats were randomly divided into 4 groups: protosappanin A group,Cyclosporin A group,control group A(heart transplant group)and control group B(no treatment).Animals were given drugs 2 days before operation.The changes of T lymphocyte subsets were measured 7 days after transplantation and myocardial pathology grade were observed.Results The pathological grade in protosappanin A group and Cyclosporin A group were significantly lower than that in control group A on the 3rd and the 7th postoperative day(P<0.01).In control group A,the ratio of CD4+/CD8+ was obviously increased compared with control group B,while protosappanin A and Cyclosporin A cound inhibit the elevation of blood CD4+/CD8+ on the 7th day after heart transplantation.Conclusion Protosappanin A shows certain immunosuppressive effect on acute rejection after heart transplantation and protective function for myocardial tissues.更多还原