【摘要】 目的:动态观察大鼠脑出血后血肿周围组织补体激活与细胞凋亡的规律。方法:用胶原酶注入到大鼠尾状核的方法制作脑出血模型。将大鼠分为脑出血、假手术组、正常组3组。采用苏木素伊红(HE)染色、免疫组织化学染色及原位末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL)分别观察各组在脑出血后第6 h、12 h、24 h、48 h、72 h、5 d、7 d时血肿周围补体C3、促凋亡基因(Bax)、抑凋亡基因(Bcl-xl)及TUNEL的表达。结果:补体C3的表达峰值在24～48 h;TUNEL、Bax蛋白表达术后12h增加,48～72 h达高峰,而Bcl-xl蛋白表达高峰在48h。结论:大鼠脑出血后血肿周围组织补体C3的表达增加与细胞凋亡的演变趋势一致,C3与凋亡有相关。更多还原

【Abstract】 Objective: To study the complement activation and apoptosis regular genes changes in the tissues of the perihematoma of intracerebral hemorrhage(ICH) in rats.Methods: Intracerebral hemorrhage was induced in rats by injection of bacterial collagenase into the caudate nucleus.Histopathological changes were studied in 6 h,12 h,24 h,2 d,3 d,5 d,7 d after the injection.The immunohistochemistry and TUNEL analysis were performed.The expression of complement factor C3,the TUNEL-positive cells,the pro-apoptotic gene expression(Bax) and the anti-apoptotic gene(Bcl-xl) were examined.Results: The expression of C3 increased to its maximum between 24-48 h.The TUNEL-positive cells and Bax protein expression increased gradually and reached the peak level between 48-72 h.The Bcl-xl protein reached the peak level at 48 h.The correlation analysis showed that the quantity of C3 was positively related to that of the TUNEL-positive cells,but the bax protein was not related to Bcl-xl protein.Conclusion: The expression of complement factor C3 may contributes to the nerve injury after cerebral hemorrhage and relate to the apotosis in the tissues surrounding the hametoma in rats.更多还原