【摘要】 目的研究亚砷酸(As2O3)对人胃癌细胞株MGC803的凋亡诱导作用及对肺耐药蛋白基因(LRP)表达的影响。方法选用人胃癌MGC803细胞系,运用体外细胞培养法,流式细胞术检测As2O3对人胃癌细胞的诱导凋亡作用;用逆转录-聚合酶链反应方法检测LRP的表达情况。结果不同浓度的As2O3均可诱导凋亡。1.0、2.0μmol.L-1的As2O3可下调LRP的表达。结论As2O3具有诱导胃癌细胞凋亡的作用,适当的浓度可下调LRP的表达。更多还原

【Abstract】 OBJECTIVE To study the apoptosis induced by arsenicals and LRP expression on human gastric carcinoma cells.METHODS FCM and RT-PCR tests were used to investigate the apoptosis levels induced by arsenic trioxide and the effect on LRP expression.RESULTS As2O3 can induce the apoptosis of human gastric carcinoma cells.1.0 μmol·L-1 and 2.0 μmol·L-1 of arsenic trioxide can decrease LRP expression.CONCLUSION As2O3 may improve apoptosis of gastric cancer and can decrease LRP expression at low concentrations.更多还原