【摘要】 目的研究淫羊藿苷对小鼠肝脏微粒体细胞色素P450(CYP)总酶含量及其亚型CYP2E1、CYP3A和CYP1A1活性的影响。方法给予小鼠ig淫羊藿苷(50、100、200 mg.kg-1.d-1),3 d后钙沉淀法制备肝细胞微粒体,UV法测定肝微粒体CYP的含量及其亚型CYP2E1与CYP3A的活性;荧光分光光度法测定肝微粒体CYP1A1的活性。结果高剂量淫羊藿苷可降低小鼠肝脏微粒体CYP的含量(约54%)、抑制CYP2E1的活性(抑制率为53.1%),对CYP3A和CYP1A1的活性无影响。结论大剂量淫羊藿苷可降低小鼠肝脏微粒体CYP的总含量,并抑制其亚型CYP2E1的活性。更多还原

【Abstract】 OBJECTIVE To investigate the effects of icariin on the contents of liver microsomal cytochrome P450 and the activities of CYP2E1,CYP3A and CYP1A1.METHODS Icariin(50,100,200 mg·kg-1·d-1) were given to male mice by ig for 3 days.Liver microsomes were prepared by calcium isolation,and the liver microsomal cytochrome P450,aniline hydroxylase(ANH),erythromycin N-demethy lase(ERD) activitiy were quantitated by UV spectrophotometry,and 7-ethoxyresorufin O-deethylase(EROD) activitiy were quantitated by fluorescent spectrophotometry.RESULTS The content of liver microsomal cytochrome P450 decreased in mice treated with icariin(200 mg·kg-1·d-1,g) for 3 days,the inhibition rate was about 54.0%(P<0.01).The catalytic activity of ANH(CYP2E1) was inhibited in mice treated with icariin(200 mg·kg-1·d-1,ig) for 3 days,and the inhibition rate was about 53.1%(P<0.01).Icariin didn’t affect the activitiy of CYP1A1 and CYP3A.CONCLUSION Icariin(200 mg·kg-1,ig) may decrease the content of liver microsomal cytochrome P450,and depress the activity of CYP2E1 in mice.更多还原