【摘要】 目的研究成都地区单纯性肥胖和正常体质量成人解偶联蛋白2基因(UCP2)Ala55Val多态性,初步探讨UCP2基因Ala55Val不同基因型与肠道菌群关系。方法采用PCR-RFLP检测86例成都地区成人(单纯性肥胖组43例,正常体质量组43例)的UCP2基因ALa55Val多态性,并分别比较不同基因型间6种肠道菌群数量的差异。结果单纯性肥胖组和正常体质量组均存在UCP2基因Ala/Ala、Ala/Val、Val/Val多态,两者表型分布差异有统计学意义(χ2=11.97,P<0.05);单纯性肥胖组UCP2等位基因突变频率高于正常体质量组,差异有统计学意义(χ2=10.06,P<0.05);单纯性肥胖组、正常体质量组和研究总体中不同基因型人群的6种肠道菌群数量差异无统计学意义(P>0.05)。结论UCP2基因ALa55Val变异可能是成都地区人群单纯性肥胖的危险因素,但该基因ALa55Val变异与肥胖的关联可能与肠道菌群数量改变无关。更多还原

【Abstract】 Objective To study on the polymorphism of UCP2 gene in Chengdu simple obesity and normal-weight people and to initially investigate the relationship between UCP2 Ala55Val variation and gut bacteria. Methods PCR-PFLP was applied to determine the genotypes of Ala55Val variant in the UCP2 gene of 86 Chengdu people (the simple obesity group,43 subjects; the normal-weight group,43 subjects). And six kinds of gut bacteria among different genotypes in different groups were analyzed. Results Both the simple obesity and the normal-weight group had the Ala55Val variants of Ala/Ala,Val/Val and Ala/Val in the UCP2 gene,and the Ala55Val genotype distributions between the two groups was significantly different (χ2=11.97,P<0.05). The allelic mutation frequency in the simple obesity group was higher than that of the normal-weight group (χ2=10.06,P<0.05). However,no significant difference was observed in the population of six gut bacteria among different genotypes in different groups (P>0.05). Conclusion The UCP2 gene mutation might be a risk factor of obesity in Chengdu area. However,this gene mutation may not be an impact factor on the alternation of gut bacteria.更多还原