【摘要】 目的观察睫状神经营养因子(CNTF)mRNA在脊髓全横断大鼠损伤后的表达变化,探讨CNTF在脊髓损伤修复中的作用。方法40只SD大鼠,随机分为假手术组和脊髓损伤组(SCI组),后者根据取材时间又分为术后1d、3d、7d和14d组。SCI组动物选择在T10节段全横断脊髓,假手术组除不横断脊髓外其余步骤与SCI组相同。动物处死前进行BBB评分评估后肢运动功能的变化。取手术部位头端和尾端0.5cm脊髓,采用RT-PCR技术检测CNTFmRNA在各组脊髓中的表达变化。结果行为学评分随着损伤时间的推移呈小幅度增加,但后肢无运动功能恢复;术后1d组和3d组横断点尾端脊髓中CNTFmRNA的表达与假手术组相比上调(P<0.05)。而7d组和14d组的表达量又逐渐恢复正常,与假手术组比较差异无统计学意义。结论脊髓损伤早期CNTF的表达变化可能与脊髓损伤修复有关。更多还原

【Abstract】 Objective To determine the expression of ciliary neurotrophic factor(CNTF)mRNA in transactional injured spinal cords and the role of CNTF in repairing and regenerating spinal cords in adult rats.MethodsForty clean adult Sprague Dawley(SD)rats were randomLy divided into sham group and operation group(SCI group).The SCI group was subdivided into four groups,with the rats sacrificed 1,3,7 and 14 days after the operations(day of postoperation,DPO),respectively.The spinal cords of the rats were completely transected at T10.The rats in the sham group underwent the same procedures as those in the SCI groups except for the transection.The Basso,Beattie and Bresnahan locomotor testing scales(BBB scores)was used to evaluate the locomotor function of hindlimbs of the rats.The expression of CNTF mRNA in the spinal cords were detected with RT-PCR technique.ResultsThe BBB scores increased gradually with the time after the spinal cord injury.But the hindlimb locomotor function did not improve significantly.The CNTF mRNA in the distal stumps of injured spinal cords increased significantly on DPO 1 and 3 compared with those in the sham group(P<0.05),and then decreased from DPO 7 and returned to normal level on DPO 14.ConclusionIncreased ciliary neurotrophic factor expression at the early stage after SCI may be associated with the repair and regeneration of the nerves in the rats.更多还原