【摘要】 采用荧光光谱、紫外-可见光谱研究了有/无金属Zn2+存在下甲基百里酚蓝(MTB)与牛血清白蛋白(BSA)的相互作用.实验结果表明,无论Zn2+离子存在与否,MTB与BSA之间均为一形成复合物的静态猝灭过程.根据Stern-Volmer方程和Lineweaver-Burk方程求出了其结合常数与热力学参数,发现Zn2+离子存在时,MTB与BSA间的作用力由静电力转为氢键和Van der Waals力作用为主,认为金属Zn2+以"离子架桥"的方式参与MTB与BSA的结合过程,从而ΔH对ΔG的贡献增大.更多还原

【Abstract】 The binding characteristics between methylthymol blue(MTB) and bovine serum albumin(BSA) with or without Zn2+ were studied by fluorescence spectra and ultra-violet spectrum.The experiments show that both the combination reactions of MTB and BSA with or without Zn2+ are a static quenching process.According to Stern-Volmer equation and Lineweaver-Burk equation,the binding constants between them and the thermodynamic parameters were obtained.It was discovered that the main binding force between MTB and BSA is electrostatic force without Zn2+,but the interaction of MTB-BSA was driven by hydrogen bonds and Van der Waals force in the presence of Zn2+.It was concluded that Zn2+ functions as "bridge ions",joining the interaction between MTB and BSA,which enhance the contribution of ΔH to ΔG.更多还原