【摘要】 首次以仿生聚多巴胺膜为功能基底膜并结合使用纳米金,构建了一种高导电性、稳健的酶生物分子固定化平台.以固定辣根过氧化物酶(HRP)为例,发展了一种新的电化学酶传感器用于H2O2的测定.结果表明,酶传感器借助聚多巴胺膜对基底电极的高结合力及其高生物亲和性与电活性,并协同纳米金的"电子通道"作用,不仅可以实现酶分子在电极表面的大量而高活性的固定化,而且能促进电子在酶活性中心和电极表面间的快速传递.与采用其它常见聚合物材料(例如壳聚糖)的酶传感器比较,以聚多巴胺/纳米金固定化平台发展的酶传感器具有更优良的检测H2O2的性能.其对H2O2的检测线性范围为4.0×10-7～4.5×10-4mol·L-1,检测限为3.7×10-7mol·L-1,灵敏度为100.2μA·L·mmol-1.此外,该酶传感器还具有优良的检测重现性和存贮稳定性,以及较好的抗干扰能力.更多还原

【Abstract】 A highly robust enzyme immobilization platform has been fabricated for electrochemical biosensors by synergistically using bio-mimetic dopamine polymer film and nanogold particles. Taking horseradish peroxidase (HRP) as an enzyme model,this presented platform has been successfully exploited to develop a novel electrochemical enzyme sensor for the detection of H2O2. Experimental results show that the nanocomposite matrix can allow HRP to be immobilized with high loading amount and well-retained bioactivity. Moreover,it can achieve rapid electron transferring between the enzyme’s active sites and the electrode surface,facilitating quick electroanalysis of H2O2. In addition,compared with other polymer materials (i.e.,chitosan),the as-proposed biosensor design using the bio-mimetic dopamine polymer can show much better analytical performances. The developed enzyme sensor can allow for the detection of H2O2 with linear concentration range of 4.0×10-7～4.5×10-4 mol·L-1,detection limit of 3.7×10-7 mol·L-1 and sensitivity of 100.2 μA·L·mmol-1. High detection reproducibility,storage stability and good interference-against ability can be additionally expected.更多还原