【摘要】 用15nm的纳米金标记羊抗人铜蓝蛋白抗体(GCP)可获得铜蓝蛋白(CP)纳米金探针(AuGCP).在pH7.8柠檬酸-磷酸氢二钠缓冲溶液中,CP与AuGCP发生特异性结合生成胶体金免疫复合物.离心分离后,离心液中的AuGCP可作为酒石酸铜(C4H4O6Cu)-葡萄糖反应体系的催化剂,生成的Cu2O微粒在620nm处有一共振散射峰.在选定条件下,620nm处共振散射信号降低值△I620nm与铜蓝蛋白浓度cCP在0.18～45ng/mL范围内存在良好线性关系,回归方程为ΔI620nm=2.27cCP+5.05,相关系数为0.9940,检出限为0.14ng/mL.该法用于人血清中铜蓝蛋白的检测,结果满意.更多还原

【Abstract】 15 nm nanogold was used to label goat anti-human ceruloplasmin(GCP) to obtain an immu-nonaogold resonance scattering(RS) probe(AuGCP) for ceruloplasmin.In the pH 7.8 citric acid-Na2HPO4 buffer solution, the immunoreaction between nanogold-labeled goat anti-human ceruloplasmin(AuGCP) and CP took place to form a nanogold-labeled immune complex.After centrifugation, the AuGCP in the cen-trifugal solutions could be used as catalyst for the reaction between Fehling reagent and glucose.The prod-uct of Cu2O particles has the strongest resonance scattering(RS) peak at 620 nm.Under the selected condi-tions, the decreased intensity ΔI620 nm is proportional to CP concentration(cCP) from 0.18 to 45 ng/mL, with a regression equation of ΔI620 nm=2.27cCP+5.05, with a correlation coefficient of 0.9940, and a detection limit of 0.14 ng/mL CP.This simple and sensitive RS assay was applied to determination of CP in human plasma with satisfactory results.更多还原