【摘要】 以4-肼基-7-硝基-2,1,3-苯并氧杂二唑肼为荧光底物,乙烯基羧酸酯、伯醇为转酯化反应底物,通过荧光分光光度法比较了几种能以固体聚合物为底物的商品化酶制剂在有机溶剂中催化转酯化反应的性质。该方法使用微量溶剂和底物进行反应,测定快捷,具有很高的直观性。实验所用4种酶制剂均可在异辛烷、甲苯和乙腈中催化转酯化反应,催化能力是脂肪酶LPL-3>蛋白酶Alcalase 3.0 T>蛋白酶subtilisin Carlsberg>蛋白酶bacillolysin,但有机溶剂的疏水性对转酯化反应影响较大,疏水性高的有机溶剂比较适合做酶催化转酯化反应的溶剂。和月桂酸乙烯酯相比,小分子的丙酸乙烯酯更有利于酶催化转酯化反应的进行。更多还原

【Abstract】 Enzymes that could catalyze transesterifaction in organic solvents were screened through fluorospectrophotometry,using 4-hydrazino-7-nitro-2,1,3-benzoxadiazole hydrazine as fluorescence substrate and vinyl ester,n-propyl alcohol as transesterification substrates.This assay format enables the rapid identification of active enzymes and is versatile for the determination of the transesterification activity of proteinases,lipases or esterases.All the four commercial enzymes used could catalyze transesterification in the organic solvents,and the order of the catalysis capability of them was lipase LPL-3>proteinase Alcalase 3.0 T>proteinase subtilisin Carlsberg>proteinase bacillolysin.Vinyl esters with smaller molecular weight and organic solvents with higher log P were more suitable for transesterification catalyzed by these enzymes.更多还原