【摘要】 目的对三氧化矿物凝聚体(MTA)、Z350纳米复合树脂、银汞合金3种常用髓腔穿孔修复材料对人牙周膜成纤维细胞(HPDLF)毒性进行体外比较研究。方法采用甲基噻唑基四唑(MTT)法研究3种材料对HPDLF增殖的影响,并采用荧光定量聚合酶链反应(PCR)法研究材料对细胞碱性磷酸酶(ALP)和骨钙素(OC)mRNA表达的影响。结果MTA的毒性最小,并能上调HPDLF中ALP mRNA和OC mRNA的表达;Z350的毒性级为1级,能够促进细胞ALP mRNA的表达,对细胞OC mRNA的表达基本无抑制;银汞合金对细胞增殖的抑制作用最大,毒性级为3级,具有中度细胞毒性,对细胞ALP mRNA和OC mRNA表达的抑制最强。结论MTA对HPDLF的毒性最小,并且能促进HPDLF的成骨功能和分化,Z350纳米复合树脂次之,银汞合金的毒性最大,并抑制牙周膜成纤维细胞的分化。更多还原

【Abstract】 Objective To select three kinds of perforation repair materials, mineral trioxide aggregate(MTA), Z350, amalgam. And to evaluate the cytotoxicity of three kinds of perforation repair materials on human periodontal ligament fibroblasts(HPDLF) in vitro. Methods The proliferation of HPDLF to three perforation repair materials were examined by methyl thiazolyl tetrazolium(MTT) assay at 1, 3 and 5 days. The mRNA expression levels of bone -associated alkaline phosphatase(ALP) and osteocalcin(OC) were determined using a real -time quantitative polymerase chain reaction(PCR). Results MTA shew almost no inhibition to HPDLF, the expression of ALP mRNA and OC mRNA in the HPDLF cultured on MTA were higher. Z350 induced a slight inhibition to HPDLF, and the expression of ALP mRNA but there was no difference in the expression of OC mRNA. Cell proliferation was significantly impaired by amalgam with grade 3, and the expression of ALP mRNA and OC mRNA were significantly reduced. Conclusion MTA have minimum cytotoxicity on HPDLF and can promote cell differentiation and regenerate of periodontal tissue. Z350 have lower cytotoxicity on HPDLF. Amalgam show highest cytotoxicity on HPDLF in the three materials and inhibit cells differentiation.更多还原