【摘要】 目的探讨兜甲蛋白(LOR)和细胞色素P450 3A5(CYP 3A5)在口腔黏膜下纤维化颊黏膜(OSF)和正常颊黏膜中的表达以及在上皮防御作用中的意义。方法首先采用免疫组化染色检测66例OSF颊黏膜和14例正常颊黏膜中LOR和CYP 3A5这2种蛋白的表达和定位,然后采用Western blot和RT-PCR方法分别在OSF患者颊黏膜和正常人颊黏膜中检测LOR和CYP 3A5的蛋白和mRNA表达。结果42例(63.6%)OSF呈LOR阳性表达,其在早、中期之间差异有统计学意义(P<0.05),而中、晚期之间差异无统计学意义(P>0.05);正常颊黏膜中CYP 3A5均呈阳性表达,5例(7.6%)OSF出现棘细胞弱阳性表达,33例(50%)出现内皮细胞弱阳性表达,与病理分期呈负相关(P<0.05)。RT-PCR显示LOR和CYP3A5mRNA在OSF与正常黏膜中表达的差异均有统计学意义(P<0.05);而Western blot检测显示,只有CYP 3A5蛋白表达在两者间差异有统计学意义(P<0.05)。结论LOR和CYP 3A5的异常表达是OSF黏膜上皮防御能力改变的表现,它们在OSF发生发展和癌变过程中可能发挥了重要作用。更多还原

【Abstract】 Objective To investigate the expression of loricrin(LOR) and cytochrome P450 3A5(CYP 3A5) in oral submucous fibrosis(OSF) and to evaluate their roles in the defending ability of epithelium mucosae. Methods The expression of LOR and CYP 3A5 was examined in the specimens of 66 OSF and 14 normal buccal mucosa samples by immunohistochemistry, and the protein and mRNA expression of them was detected by Western blot and reverse transcriptase-PCR(RT-PCR). Results LOR was overexpressed in 42(63.6%) cases of OSF, and showed a significant difference only between the early and moderately stages of OSF(P<0.05), but no clear difference between moderately and advanced stages(P>0.05). All normal buccal mucosa tissues showed positive immunoreactivity for CYP 3A5 protein in the membrane and cytoplasm of spinous epithelial cells and cytoplasm of endothelial cells, 5(7.6%) cases of OSF showed weak staining of CYP 3A5 in spinous epithelial cells and 33(50%) showed faint in cytoplasm of endothelial cells. A negative relationship between its expression and pathological stages was found in OSF(P<0.05). RT-PCR results were fully consistent with the immunohistochemical data. But the results of Western blot only showed the ex- pression of CYP 3A5 was significantly higher in normal buccal mucosa samples than OSF. Conclusion The results suggest that the LOR and CYP 3A5 might play a vital role in the change of defending ability of epithelium mucosae as well as the pathopoiesis and carcinogenesis of OSF.更多还原