【摘要】 蛋白质糖基化是一种重要的翻译后修饰,糖基化对蛋白质的结构和功能有着重要的影响。目前,作为蛋白质组学的一个组成部分,糖蛋白质组学是备受关注的研究热点,而从复杂的生物样品体系中富集糖蛋白/糖肽是蛋白质糖基化研究的重点和难点。本文就糖蛋白/糖肽分离富集方法的研究进展和应用作了简要概述。这些方法包括常用的凝集素亲和法、硼酸法、肼化学法和亲水作用法,还包括分子筛法、强阳离子交换法等新方法。更多还原

【Abstract】 Protein glycosylation as an important post-translational modification has a significant effect on the structures and functions of proteins.As a section of proteomics,glycoproteomics is in the spotlight currently,and the efficient separation and enrichment of glycoproteins/glycopeptides from complex biological samples is the key and difficult point of glycoproteome research. In this paper,the progress in methods for the separation and enrichment of glycoproteins/glycopeptides and their applications are overviewed. These methodologies not only include most-often-used methods such as lectin-based affinity chromatography,boronic acid method,hydrazide chemistry and hydrophilic chromatography,but also some novel methods,for example,size exclusion chromatography and strong cation exchange enrichment.更多还原