【摘要】 采用反相高效液相色谱法测定落叶松中二氢槲皮素的含量。确定色谱条件为:色谱柱Hypersil ODS-C18(4.6mm×200mm,5μm);流动相为V(甲醇):V(水):V(冰醋酸)=40:59:1;流速为1.0mL.min-1;检测波长为288nm;柱温为28℃。在此条件下,二氢槲皮素与其它组分得到良好的分离。线性范围为0.02～0.2μg.μL-(1r=0.9998),精密度RSD为0.96%(n=5),平均回收率为100.4%。更多还原

【Abstract】 The method of determination dihydroquercetin in Larch by HPLC was established in this thesis.The following HPLC conditions are the best:column:Hypersil ODS-C18(4.6 mm×200 mm,5μm);mobile phase:V(methano1):V(wate)r:V(glacial acetica acid)= 40:59:1,flow rate:1.0 mL.min-1;detection wavelength:288 nm;temperature:28℃.Dihydroquercetin separated thoroughly from other components on this condition,the linearity of Dihydroquercetin was obtained within the range of 0.02 μg.μL-1(r=0.9998),the average recovery was 100.4% with relative standard deviations of 0.96 %.更多还原