【摘要】 采用单细胞凝胶电泳技术(single cell gel electrophoresis,SCGE),又称彗星电泳,表征不同剂量α,γ射线照射诱导健康人体外周血淋巴细胞DNA和小鼠活体与离体外周血淋巴细胞DNA瞬时损伤的剂量效应关系,建立了α,γ射线辐照剂量刻度曲线,并对活体小鼠的辐射损伤进行了吸收剂量估算,对辐射危害进行了评价。结果表明,单细胞凝胶电泳技术能够精确表征射线所致生物体损伤的生物学效应,而且能够对辐照后生物体的吸收剂量、尤其是辐射事故后的生物体进行剂量离线评测,建立生物体辐射损伤事后评测机制。更多还原

【Abstract】 The transient irradiation-caused DNA damage in the human peripheral blood lymphocytes,mouse peripheral blood lymphocytes and alive mouse irradiated by α-ray and γ-ray was investigated,and the single cell gel electrophoresis(SCGE,Comet Assay) was used to detect the extent of DNA damage.On this basis,the dose-effect curve and the evaluating method for radiant after-effect were established,the absorbed dose of alive mouse A irradiated by γ-rays was computed.The results indicate that not only the dose-effect can be described by using SCGE,but also the dose-computed after organism irradiated by radiant rays is achieved with it,and SCGE may be used as a new biological dosimeter.更多还原