【摘要】 在优化的实验条件下,利用电化学方法制备了甘氨酸修饰电极,对修饰膜的电活性进行了表征。用循环伏安法研究了鸟嘌呤(G)和8-羟基脱氧鸟嘌呤核苷(8-OH-dG)在聚甘氨酸修饰电极上的电化学行为,并建立了对两者进行分别检测和同时检测的分析方法。实验结果表明,聚甘氨酸修饰电极可以增强鸟嘌呤和8-羟基脱氧鸟嘌呤核苷在电极表面的吸附,并且可以加快鸟嘌呤和8-羟基脱氧鸟嘌呤核苷在电极表面的电子传输,使两种电活性物质在聚甘氨酸修饰电极上的电化学信号明显增大,检测灵敏度大大提高,并且该修饰电极具有良好的稳定性和重现性,可用于鸟嘌呤和8-羟基脱氧鸟嘌呤核苷的分别和同时检测。更多还原

【Abstract】 In this paper, a polyglycine modified electrode was prepared by electrochemical polymerize glycine under the optimized experimental conditions. The electric activity of modified membranes was characterized. And the electrochemical behavior of guanine (G) and 8-hydroxy-deoxy guanosine (8-OH-dG) were studied with CV technique at the modified electrode. We set up an analytical detection method to determine guanine and 8-OH-dG separately and simultaneously. Experimental results show that the polyglycine modified electrode can enhance the adsorption of guanine and 8 -OH -dG and speed up the electronic transmission at the electrode surface which contribute the electrochemical signal increases markedly, and the detection sensitivity improved either. The modified electrode gives good stability and reproducibility, which good factors give a satisfied result in determination of guanine and 8-OH-dG respectively and simultaneously.更多还原