【摘要】 目的:探索选择性增菌在痰标本TEM型ESBL菌株痰标本检测中应用的可行性。方法:从郑州大学第一附属医院30份不重复的痰标本中分离出TEM型ESBLs菌株,采用LB液体培养基进行增菌培养,应用氨苄青霉素(终质量浓度为100mg/L)进行选择抑菌,采用酶抑制剂增强试验及PCR技术进行ESBLs确证。结果:酶抑制剂增强试验中头孢噻肟与头孢噻肟/克拉维酸抑菌环直径差大于5mm;PCR扩增产物在约900bp处有明显条带。结论:选择性增菌方法简单、快速,准确,可用于痰标本中TEM型ESBL菌株的检测。更多还原

【Abstract】 Aim:To investigate the application of select cloning in the detection of TEM-type ESBL in sputum.Methods:30 specimens which contained ESBLs from the First Affiliated Hospital of Zhengzhou University were collected.Cloning was selected by LB culture medium which contains Amp(100 mg/L).Detecting ESBL by enzyme inhibitor enhancing test and PCR.Results:The diameter size differences between CTX and CD03 exceeded 5 mm.The PCR amplying product was about at 900 bp in agarose electrophoresis.Conclusion:Select cloning is rapid, accurate and can be used in detecting ESBL in sputum.更多还原