【摘要】 目的:探讨DNA甲基转移酶1(DNMT1)在宫颈癌发生发展中的作用。方法:应用原位杂交技术检测52例宫颈癌、60例宫颈上皮内瘤变和20例正常宫颈组织DNMT1 mRNA的表达;分析DNMT1 mRNA的表达和宫颈癌患者临床病理指标的关系。结果:正常宫颈、宫颈上皮内瘤变和宫颈癌组织中DNMT1 mRNA的阳性率分别为10.0%(2/20)、63.3%(38/60)和78.8%(41/52),呈升高趋势(χ2=29.057,P<0.05),宫颈上皮内瘤变和宫颈癌组织DNMT1 mRNA的阳性率高于正常组织。不同宫颈癌的病理类型、临床分期、组织学分级及有无淋巴结转移组间DNMT1 mRNA阳性率差异无统计学意义。结论:DNMT1参与了宫颈癌的发生,并在宫颈上皮内瘤变时就已经起了重要作用。更多还原

【Abstract】 Aim:To study the relationship between DNMT1 expression and oncogenesis of uterine cervical carcinoma.Methods:The expression of DNMT1 mRNA was detected by in situ hybridization in 52 cases of cervical carcinoma,60 cases of cervical intraepithelial neoplasia(CIN) and 20 cases of normal cervical tissue.Results: The positive rate of DNMT1 mRNA expression in cervical carcinoma and CIN were 78.8% and 63.3%, higher than that(10.0%) in normal cervical tissue(P<0.05).No relationship was observed between DNMT1 mRNA and pathological categories, clinical stages, histological grades or lymph node status(P>0.05).Conclusion: Progressively increasing expression of DNMT1 is associated with cervical carcinogenesis even during the precancerous stages.更多还原