【摘要】 目的:研究肺癌组织中希佩尔林道(VHL)和缺氧诱导因子-1α(HIF-1α)的表达并探讨其临床意义。方法:将80例肺癌及20例正常肺组织手术切除标本常规制作石蜡包埋切片,采用免疫组化EnVisionTM二步法测定VHL和HIF-1α的表达。分析VHL和HIF-1α的表达与临床分期、分化程度、有无淋巴结转移间的关系。结果:肺癌组织VHL表达阳性率(56.3%)明显低于正常肺组织(90.0%)(P<0.01);HIF-1α表达阳性率(65.0%)明显高于正常肺组织(20.0%)(P<0.01);中高分化、Ⅰ～Ⅱ期、无淋巴结转移的肺癌组织VHL表达阳性率明显高于低分化和未分化、Ⅲ～Ⅳ期及有淋巴结转移者(P<0.01～0.05),而HIF-1α表达阳性率则相反(P<0.05);肺癌组织中VHL和HIF-1α表达水平呈高度负相关(P<0.01)。结论:VHL和HIF-1α可能是反映肺癌发生、进展、临床生物学行为及预后的重要标志物。更多还原

【Abstract】 Objective To investigate the expression of von Hippel-Lindau(VHL)protein and hypoxia inducible factor-1α(HIF-1α)in the lung cancer tissue and to detect their clinical significance.Methods EnVisionTM immunohistochemistry was used for detecting the expression of VHL and HIF-1α in routinely paraffin-embedded sections from the specimens of lung cancer(n=80)and normal lung tissues(n=20).We also analyzed the relation between the expression of VHL and HIF-1α and the clinical stage,differentiation,and with or without lymphatic metastasis.Results The positive rate of VHL was significantly lower in lung cancer(56.3%)than that in normal lung tissues(90.0%)(P<0.01).The positive rate of HIF-1α was significantly higher in lung cancer(65.0%)than that in normal lung tissues(20.0%)(P<0.01).The positive rate of VHL was significantly higher in the middle and high-differentiated,StageⅠ～Ⅱ,and no-metastasis of lymph node lung cancer tissues than that in the poorly-differentiated,Stage Ⅲ～Ⅳ,metastasis of lymph node lung cancer tissues(P<0.01～0.05).But the expression of HIF-1α in the lung cancer tissues was just the opposite as compared with that of VHL(P<0.05).VHL and HIF-1α expression levels showed highly negative correlation(P<0.01).Conclusion The expression of VHL and HIF-1α may be important biological markers for carcinogenesis,progression,clinical biological beha-viors,and prognosis of lung cancer.更多还原