【摘要】 目的:研究肺部良恶性病变组织中泛素( ubiquitin)和cullin-1 ( cul-1 )的表达及其临床病理意义。方法:将80例肺癌和20例肺部良性病变手术切除标本常规制作成石蜡包埋切片,采用免疫组织化学EnVsionTM二步法测定泛素和cul-1的表达。分析泛素和cul-1表达与临床分期、分化程度、有无淋巴结转移间的关系。结果:肺癌组织中泛素和cul-1表达阳性率( 51.3%, 60.0%)明显高于肺良性病变组织(20.0%,30.0%;P<0.05) ,中高分化、Ⅰ～Ⅱ期和无淋巴结转移肺癌组织中泛素和cul-1表达阳性率均明显低于低分化和未分化、Ⅲ～Ⅳ期和有淋巴结转移者(P<0.01 ～0.05 )。肺癌组织中泛素与cul-1的表达存在高度一致性(χ2=4.04,P<0.05 )。结论:泛素和cul-1表达可能与肺癌发生、进展、临床生物学行为和预后存在密切关系。更多还原

【Abstract】 Objective To investigate the expression of ubiquitin and cullin-1(cul-1) in benign and malignant lesions of the lung and to determine their clinicopathological significance.Methods EnVisonTM immunohistochemistry was used to detect the expression of ubiquitin and cul-1 in the conventional paraffin-embedded sections from the specimens of lung cancer(n=80) and benign lesion tissues of the lung(n=20).We also analyzed the relation of the expression of ubiquitin and cul-1 with the clinical stage,differentiation,and with or without lymphatic metastasis.Results The positive rates of ubiquitin and cul-1 were significantly higher in lung cancer(51.3% and 60.0%) than those in benign lesion tissues of the lung(20.0% and 30.0%;P<0.05).Positive rates of ubiquitin and cul-1 were all significantly lower in the middle and high-differentiated,StageⅠ～Ⅱ,and no lymphatic metastasis patients with lung cancer than those in no-or low-differentiated,Stage Ⅲ～Ⅳ,and lymphatic metastasis patients with lung cancer tissues(P<0.01～0.05).High consistency was found between the positive expression of ubiquitin and cul-1 in lung cancer tissues(χ2=4.04,P<0.05).Conclusion Expression of ubiqui-tin and cul-1 in lung cancer tissues may be closely related to the carcinogenesis,progression,clinical biological behaviors,and prognosis of lung cancer.更多还原