【摘要】 采用不同剂量的60Co γ射线辐照糖多孢红霉菌M孢子,并采用不同的菌种保护剂,以研究60Co γ射线对糖多孢红霉菌M的诱变效应。结果表明,使用20%甘油吐温保护效果较好,可作为诱变保护剂;当辐照剂量为400Gy时,致死率为90%左右可作为适宜诱变剂量。诱变处理后利用链霉素抗性突变选育得到1203株链霉素抗性突变株。其中5株形态发生较明显的变化,形态变异率为4.16‰;2株突变株的发酵液具有抑菌活性,正突变率为1.67‰。更多还原

【Abstract】 Spores of Saccharopolyspora erythraea M with different protective agents were treated with different doses of ~ 60 Co γ-rays irradiation, and their morphological variations were observed and mutants were selected. The results indicated that 20% glycerol (contain 0.1% tween-20) showed a better protective effect to irradiation, and lethal ratio reached 90% when irradiation dose at 400 Gy, therefore 400 Gy was selected as the appropriate dose. 1203 strains were obtained by streptomycin resistance screening, among them, 5 strains changed in their phenotype and 2 strain fermentation products had antibacterial activity. The morphological variation rate was 4‰ and positive mutation rate was 1.67‰.更多还原