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卫星搭载处理丹参种子SP2代的SRAP分析
SPACE FLIGHT EFFECTS ANALYSIS OF SP2 Salvia Miltiorrhiza Bge.USING SRAP MARKER
【摘要】 为探讨航天搭载丹参种子的诱变效应,本研究调查了航天搭载处理对SP2代材料植株地上、地下部各性状的影响,并利用SRAP技术分析其生物学水平上的差异。结果表明:丹参种子SP2代的航天诱变效果明显,不同植株不同性状所表现出的效应不一致。利用12对引物对丹参航天材料进行分析,共产生146条多态性条带,丹参航天材料之间的遗传相似系数在0.453～0.867之间,平均为0.648。
【Abstract】 In order to study the effects of space mutation of Salvia miltiorrhiza Bge., agronomic traits had been studied in SP2 by field investigation and SRAP. The results showed that the mutagenic effects of space flight were complicated and there were variant effect on different plants and different traits.146 alleles had been detected from 19 materials with 12 primer pairs. Jaccard’s similarity coefficient ranged from 0.453～0.867, the average was 0.648.
【基金】 国家航天工程项目(2006HT10-0165);山东省良种工程项目(鲁科农社字[2008]167号);山东省农科院高技术自主创新基金项目(2007YCX012);山东省农科院博士基金项目(2006YBS003)
- 【文献出处】 核农学报 ,Journal of Nuclear Agricultural Sciences , 编辑部邮箱 ,2009年05期
- 【分类号】S567.53
- 【被引频次】13
- 【下载频次】181