【摘要】 以草莓品种星都2号和全明星的无菌苗叶盘和叶柄基部为外植体,采用携带美洲拟鲽编码pre、pro和mature3种抗冻蛋白(AFP)基因的双元载体系统的根癌农杆菌进行转化,获得7个Km抗性株系。经PCR扩增及PCR-Southern杂交检测,6个株系呈阳性,结果表明,编码pre、pro和mature的AFP基因分别整合到了星都2号和全明星染色体DNA中,其中获得pre、pro和mature转星都2号株系各1个,转化率分别为1.56%、1.49%和1.67%;mature基因转全明星株系3个,转化率为4.05%。更多还原

【Abstract】 Antifreeze protein(AFP) of Pseudopleuronectes americanus gene encoding pre,pro and mature was introduced into strawberry(Fragaria × ananassa cv. Xingdu 2 and Allstar) by mediated Agrobacterium gene transfer of a binary vector,and the axenic leaf discs and lower part of petioles of strawberry were used as explants.The results showed that the foreign AFP gene encoding pre,pro and mature was integrated independently into the genome of six transgenic strawberry plants by PCR and PCR-Southern bloting analysis of seven Kmamycin-resistant transformed plants.Three regenerated Xingdu 2 lines were positive for pre,pro and mature,and their transformation efficiency was 1.56%,1.49%and 1.67%respectively.Three regenerated Allstar lines were positive for mature and its transformation efficiency was 4.05%.更多还原