【摘要】 采用RT-PCR技术克隆了玉米基因sbe1全长cDNA序列,在NCBI上序列比对后与文献报道的sbe1基因序列同源性达到99%。同时构建了原核生物表达载体pET32(a+)-sbe1,成功表达出了SBEⅠ蛋白。为进一步体外研究SBEⅠ的物理化学性质及催化机理奠定基础。更多还原

【Abstract】 The cDNA of sbe1c from maize was cloned by RT-PCR and compared with the reported sequence of sbe1 cDNA in GenBank.The results showed that the nuleotide sequence homology were 99%.The sbe1 cDNA were inserted pET32a(+) vector by restriction endonuclease digestion,electrophoresis,gel reclamation DNA ligation and so on molecular biological technologies,and that which were transformed to E.coil BL21(DE3).At the same time sbe1 cDNA was induced expression in E.coil BL21(DE3) by IPTG,and the SBEⅠprotein was expressed.The experimental result lay a foundation for further study on the physical and chemical properties of SBEⅠ in vitro.更多还原