【摘要】 比较了5种大孔吸附树脂对黑米皮花色苷(Black Rice Anthocyanins,BRA)的吸附纯化效果,研究了AB-8型大孔树脂对BRA的吸附与解吸特性.结果表明:AB-8大孔树脂对BRA具有较好的吸附和解吸能力,是吸附纯化BRA的最佳树脂类型,其分离纯化BRA的最佳工艺参数为:上柱液pH值=2,样品质量浓度1.0 mg/mL,吸附流速1.0 mL/min,以体积分数为70%乙醇为解吸剂,洗脱速度为1.0 mL/min.树脂的重复利用率好,使用5次后吸附率无显著性差异(P>0.05),使用7次后,吸附率仅降低2.58%.BRA经AB-8大孔树脂纯化后,花色苷含量提高2.38倍,总抗氧化能力提高3.99倍.更多还原

【Abstract】 The absorption and purification effects of 5 kinds of resins on Black Rice Anthocyanins(BRA) were compared,and the properties of adsorption and desorption for AB-8 on BRA were further investigated.The result shows that AB-8 is the best resin for BRA due to its better adsorption and desorption capability.The optimal technical parameters of AB-8 are as follows: the pH of solution is 2.0;the anthocyanins concentration of the sample solution is 1.0 mg/mL,and the adsorption flow rate is 1mL /min;the desorption flow rate is 1.0mL/min with 70% ethanol as the desorptioner.The resin has a good reutilization.The desorption capability has no significant changes after using 5 times(P>0.05),and with only 2.58% reduction after using 7 times.After purified with AB-8,the content of BRA increases 2.38 times and total antioxidant capability enhances 3.99 times.更多还原