【摘要】 参考GenBank发表的猪繁殖与呼吸综合征病毒(PRRSV)的nsp2基因序列,在nsp2大缺失区下游的保守区设计了1条下游引物,在小缺失区设计1条上游引物,用于变异株的扩增。在大缺失区内部设计1条上游引物,与变异株共用下游引物,用于普通株的扩增,建立了PRRSV变异株与普通株二重RT-PCR鉴别方法。建立的RT-PCR方法用于临床病料的检测,部分阳性结果测序,变异株序列与报道的序列同源性在97%以上。结果表明,该方法特异、快速、简便,可以用于PRRSV变异株和普通株的鉴别。更多还原

【Abstract】 According to the nsp2 gene sequence of PRRSVs in GenBank,a downstream primer was designed in the conserved region of the downstream of large deletion of nsp2 and a upstream primer in the short deletion to amplify the variant strain.Common strain was amplified using a upstream primer within the large deletion and the same downstream primer to variant strain.A duplex RT-PCR distinguish method to differentiate the PRRSV variant and common strains was development.This method was applied to test clinic samples.Part positive results were sequenced and showed more than 97% homologous with the reported sequences.These results showed that this method was specific,rapid and simple.This method could be applied to differentiate the PRRSV variant and common strains.更多还原