【摘要】 PCR扩增猪瘟病毒(CSFV)定点突变后的E2基因,克隆至原核表达载体质粒pET-28a中,重组质粒pET28a-E2转化BL21(DE3),IPTG诱导,高效表达了E2基因,表达量达菌体总蛋白的25.3%。免疫印迹表明,所表达的蛋白是CSFV特异性的。更多还原

【Abstract】 A mutated CSFV E2 gene was amplified by PCR and cloned into pET-28a to construct the prokaryotic expression vector pET28a-E2.The recombinant plasmid was transformed into BL21(DE3) and expressed at high level induced by IPTG.The expressed E2 protein was amounted to 25.3% of the total bacteria proteins.The result of Western-blot showed that the recombinant E2 protein was CSFV-specific.更多还原