【摘要】 以抗旱性不同的玉米品种农大108(抗旱性较强)和掖单13(抗旱性较弱)为材料,经过亚精胺(Spd)浸种处理,研究在聚乙二醇(PEG)-6000渗透胁迫下,幼苗根尖线粒体ATPase水解活性与膜上非共价结合态多胺含量的关系。结果表明,渗透胁迫条件下,抗性强的农大108的ATPase活性下降的幅度明显小于抗性弱的品种掖单13,而农大108的线粒体膜上非共价结合态亚精胺的上升幅度明显大于掖单13。亚精胺浸种处理,明显增强了掖单13幼苗的抗性,促进了掖单13在胁迫条件下非共价结合态Spd含量的增加,同时抑制其在胁迫条件下ATPase活性的降低。这些结果表明,亚精胺浸种处理,通过提高玉米幼苗根尖线粒体膜上的非共价结合态Spd含量,维持ATPase活性的稳定,从而提高玉米幼苗的抗渗透胁迫能力。更多还原

【Abstract】 Two maize(Zea mays L.)cultivars,Nongda 108(drought-tolerant) and Yedan 13(drought-sensitive) were selected to investigate the effects of seed soaking with spermidine on ATPase activity and conjugated-polyamine contents in mitochondria membrane of seedling roots under osmotic stress.The result showed that,in 7 days after polyethylene glycol(PEG)-6000 treatment,the ATPase activity decreased more in Yedan 13 than in Nongda 108,while the content of noncovalently conjugated-spermidine(Spd) increased more in Nongda 108 than in Yedan 13.Under osmotic stress,seed soaking with spermidine could obviously increase the noncovalently conjugated-Spd level and inhibit the decrease of ATPase activity in Yedan 13,so as to enhance its tolerance to stress.The results suggested that Spd seed-soaking could enhance the tolerance of maize seedlings to osmotic stress,especially for the drought-sensitive cultivar,by increasing the noncovalently conjugated-Spd level and maintaining the stability of ATPase activity in mitochondria membrane.更多还原