【摘要】 利用猪瘟弱毒疫苗(C株)免疫新西兰大白兔,制备抗猪瘟高免血清IgG。运用间接ELISA方法测血清效价,再经饱和硫酸铵盐析沉淀法提纯血清IgG,SDS-PAGE电泳法鉴定提纯IgG的纯度,结合无菌检查试验和仔猪接种试验,检测血清IgG的安全性。结果表明,获得了蛋白含量为6 mg/mL,效价为1∶6400的高效价、安全性高、成本低的抗猪瘟高免血清IgG。为猪瘟病毒的检测和猪瘟的防制提供了理想的生物制剂。更多还原

【Abstract】 To prepare hyperimmunized rabbit serum against classical swine fever virus(CSFV) C strain,the Zelanian rabbit were immunized with the attenuated CSFV vaccine.The serum titer of immunized rabbit was determined by indirect ELISA and the IgG was purified by saturated ammonium sulfate precipitation.Then,the purity of IgG was checked by SDS-PAGE electrophoresis and the safety was confirmed by microorganism test and inoculating piglets.The results showed that the protein concentration of the purified IgG was 6 mg/mL,and the ELISA titer was 1∶6400.Taken together,we have produced serum IgG against CSFV,which shows high titer,high safety,low cost,and can be used to treat or prevent swine fever.It is an ideal biological agent for the detection of CSFV and the control of CSF.更多还原