【摘要】 运用RT-PCR法,从小麦叶片中克隆出SOD、CAT、APX、GR、CBF、GI等几个抗冷相关基因的cDNA片段,序列比对结果显示,这些基因与GenBank上已登录的大麦、小麦、玉米、水稻等相关基因的同源性较高,表明克隆出了上述几个小麦抗冷相关基因,其中的APX和GR为普通小麦中首次报道。更多还原

【Abstract】 Partial cDNA sequences of superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),glutathione reductase(GR),C-repeat bing factor(CBF) and GIGANTEN(GI) were isolated from wheat leaves using RT-PCR.Sequence analysis showed that these genes had high identity to relevant genes from wheat,barley,maize and rice registered in GenBank,im-plying that these cloned fragments were really the cold-tolerant genes.Among them,APX and GR were firstly isolated from common wheat(Triticum aestivum L.).更多还原