【摘要】 <正>1材料与方法1.1 材料共采集血样103份,其中,44份河曲藏獒(HTM),42份青海藏獒(QTM),5份青海藏狮犬(QTS)以及12份青海土种犬(NAD)。均采集后肢静脉肝素钠抗凝血5～10 ml,24 h内带回实验室,处理后置低温冰箱( -40℃)冻存备用。更多还原

【Abstract】 [Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff,and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. [Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff,Qinhai Tibetan Mastiff,Tibetan Spaniel and native dogs of Qinghai. Seven blood protein locus(Tf,Po,Sα2,Hb,Alb,Pr and Amy)were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [Result] Genetic variations were observed in Tf,Sα2 and Po in four populations,others were not polymorphic. There were three alleles at the locus of Tf and Po,two alleles at the loci of Sα2. Effective number of alleles and Nei’s average expected heterozygosity were 1.532 4 and 0.230 3 relatively,all higher in Tibetan Mastiff than other populations. [Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation.更多还原