【摘要】 以8个烤烟栽培品种为试验材料,对烟草基因组DNA的提取方法进行比较,并对建立烟草SRAP-PCR反应体系的影响因子设置梯度试验.结果显示:改进的CTAB法能较好地满足烟草基因组DNA的提取,在20μLSRAP-PCR的反应体系中,DNA模板40ng、Mg2+1.5mmol/L、dNTP0.25mmol/L、引物0.2μmol/L、Taq聚合酶1.5U,8个烤烟品种的扩增多态性高,带型清晰,差异明显.更多还原

【Abstract】 Method of DNA extraction and factors influencing SRAP analysis were studied using eight tobacco cultivars.The results showed that,improved method of CTAB contributed to a better extraction of genomic DNA of tobacco.A reliable PCR reaction system for detecting SRAP was developed,each 20 μL PCR reaction mixture consisted of 40 ng of genomic DNA,0.25 mmol/L of dNTPs,1.5 mmol/L of Mg2+,0.2 μmol/L of primer and 1.5 unit of Taq polymerase,every amplification out of the eight cultivars was effective,clear and their differences were significant.更多还原