【摘要】 运用隐马尔柯夫模型(Hidden Markov model,HMM),对粳稻Oryza sativaL.ssp.japonica的蛋白质数据库进行搜索,共找到192个MYB蛋白同源序列,其中有126个R2R3-MYB蛋白,6个R1R2R3-MYB蛋白以及60个MYB相关蛋白(MYB-related protein).进一步分析所有粳稻的MYB蛋白基因在染色体上的分布,结果发现,MYB基因基本上是成簇分布的,在染色体1~8号特别明显.运用基于EM(Expectation maximization)算法的MEME程序,对MYB蛋白的功能域进行多序列比对分析,确定了R1、R2与R3结构域在每个位置上最大可能氨基酸的频率.此外,还对水稻中的MYB蛋白家族作了初步的进化分析.更多还原

【Abstract】 192 MYB proteins were identified in rice genome by searching the Oryza sativa L.ssp.japonica protein database using Hidden Markov model(HMM) scan strategy.Among them,126 were R2R3-MYB,6 were R1R2R3-MYB,and 60 were MYB-related.Further studies showed that the MYB genes were not randomly distributed in chromosomes,but clustered in chromosomes,especially in chromsome 1,2,3,4,5,6,7 and 8.Multiple alignment was carried out with MYB proteins using MEME method and the most favored amino acid at each site of R1,R2 and R3 domain were determined.In addition,a preliminary analysis for evolution of the rice MYB protein family also was performed in this paper.更多还原