【摘要】 应用PCR方法,获得了分离自广东番茄、茄子、辣椒、烟草、空心菜、沙姜、姜、马铃薯、花生、菊花、桑树和藿香共12种作物21个茄科雷尔氏菌菌株的16S rDNA.序列测定及比较结果表明,21个广东茄科雷尔氏菌菌株16SrDNA近全长序列均为1 528 bp,序列间同源率99.2%~100%;各菌株的16S rDNA序列间只有1~12个不等的碱基差异,其中20个菌株的458~460位及474位的碱基是ACT和T,而菌株HZ-1则是TTC和A,说明广东茄科雷尔氏菌的16S rDNA序列十分保守.系统进化分析结果显示,仅菌株HZ-1聚类于茄科雷尔氏菌2 a亚组中,其余20个菌株均聚类于茄科雷尔氏菌区组1中.更多还原

【Abstract】 16S rRNA genes of twenty-one strains of Ralstonia solanacearum were amplified by PCR,which were isolated from Lycopersicum esculentum,Solanum melongana,Nicotiana tabacum,Capsicum annuum,Ipomoea aquatica,Kaempferia galangal,Zingiber officinale,Solanum tuberosum,Arachis hypogaea,Dendranthema morifolium,Morus alba and Herba agastaches,respectively.All of the nearly complete 16S rRNA gene sequences of the strains were determined to be 1 528 bp,and sequence identity of the 16S rDNA ranged from 99.2% to 100%.The number of different base-pair ranged from 1 to 12 bp among 16S rDNA.The specific nucleotides at positions 458-460 and 474 of 16S rDNA were ACT and T respectively,except that the isolate HZ-1 were TTC and A.The results indicated that the 16S rDNA sequences of R.solanacearum from Guangdong were highly conserved.Phylogenetic analysis of 16S rDNA sequences showed that HZ-1 clustered to subdivision 2a,while other 20 isolates clustered to division 1.更多还原