【摘要】 试验采用ELISA法对离体水牛卵巢不同大小卵泡液中的雌二醇(E2)和睾酮(T3)水平进行了定量分析。结果表明:较大卵泡(直径≥6 mm)液中雌二醇的平均浓度是中小卵泡(直径为2~5 mm)液中的8.5倍;而中小卵泡液中睾酮的平均浓度则是较大卵泡液中的3.3倍;较大卵泡液中雌二醇与睾酮比值显著高于中小卵泡。试验结果说明高浓度的雌激素对卵泡的发育有促进作用,而高浓度的雄激素则发挥抑制作用。更多还原

【Abstract】 Concentration of estradiol(E2) and testosterones(T3) in deferent volume follicular fluid of swamp buffalo were mearsuered by ELISA.The results showed that,the mean concentration of E2 in follicular fluid of large follicle(d≥6 mm) is 4.11 times(2 209.151 pg/mL vs 537.528 pg/mL,P<0.01)higher than that in small follicles(d=2～5 mm).But the mean concentration(5.103 ng/mL)of T3 in follicular fluid of small follicle is 3.42 times than that in large follicles(1.493 ng/mL,P<0.01).Simultaneity,E2/T3 in large follicular fluid(131.470)is significantly higher than that in small follicles(0.166,P<0.01).In conclusion,the higher concentration of estrogen promotes follicule development,but the higher concentration of T3 inhibits.更多还原